Organized by:
Promega Corporation and the University of Michigan Center for Chemical Genomics
Promega Corporation and the
Emory Chemical Biology Discovery Center

Event Details:*
Friday, March 25, 2011
This event has already occurred, Click Here for photos from the event.
Lecture only: 10:00 a.m. - 12:45 p.m. (Full)
Lecture and lab: 8:30 a.m. - 5:00 p.m. (Full)
Lunch provided

*The lecture and lab are free of charge, however,
registration is required to guarantee your place.

Laboratory Workshop Location:
Emory Chemical Biology Discovery Center
Dental Building
1462 Clifton Rd. NE, Rm 307
Atlanta, GA 30322

Lecture Location:
Emory University
Rollins Research Center
1510 Clifton Rd. NE, Rm 2052
Atlanta, GA 30322

Here is WHAT you'll learn:

  • How to build a powerful cell-based or biochemical assay using bioluminescence detection
  • Hands on practical work to see how reporter gene assays can be used to study cell signaling processes; as an example we will run two different labs:
  • Gene regulation biomarker: measure NfkappaB activation in HEK293 cells
  • Second messenger biomarker: measure cAMP changes via GPCR activation
  • Tips and tricks-of-the-trade for obtaining the most useful data from cell-based reporter assays
  • How to adapt a cell-based assay for high-throughput screening
  • Details of the Emory Chemical Biology Discovery Center (ECBDC), its capabilities and how they could apply to your research
  • Project selection process for submitting HTS and probe design requests to the ECBDC

Agenda: Lecture Only

Time Topic
10:00 – 10:10 am Welcome and Introduction to the Lecture Section:
Haian Fu, Ph.D., Director, Emory Chemical Biology Discovery Center; and Professor of Pharmacology and Hematology/Medical Oncology, Emory University
10:10 – 11:00 am "Using Luminescent Technology to Monitor Key Cell Signaling Biomarkers"
Frank Fan, Ph.D., Director of Research, Promega Corporation
11:00 – 11:40 am "Screening for Small Molecule Modulators at the Emory Chemical Biology Discovery Center"
Yuhong Du, Ph.D., Asssociate Director of HTS and Assay Development, Emory Chemical Biology Discovery Center; and Assistant Professor of Pharmacology, Emory University
11:45 – 12:45 pm Tour of the Emory Chemical Biology Discovery Center
Clinton Maddox, Research Scientist, Emory Chemical Biology Discovery Center, Emory University
11:45 – 12:45 pm Lunch (to be provided)

Agenda: Lecture and Lab

Time Topic
8:30 – 8:45am Introduction & Welcome:
Jeffrey L. Kelly, Ph.D., Strategic Marketing Manager, Cell Analysis, Promega Corporation
8:45 – 10:00am GloResponse NFkappaB Laboratory Activity, Part I
Trista Schagat, Ph.D., Scientific Applications Manager, Promega Corporation; and Adjunct Research Investigator, Department of Biological Chemistry, University of Michigan Medical Center
10:00 – 10:10am Welcome and Introduction to the Lecture Section:
Haian Fu, Ph.D., Director, Emory Chemical Biology Discovery Center; and Professor of Pharmacology and Hematology/Medical Oncology, Emory University
10:10 – 11:00am "Using Luminescent Technology to Monitor Key Cell Signaling Biomarkers"
Frank Fan, Ph.D., Director of Research, Promega Corporation
11:00 – 11:40am "Screening for Small Molecule Modulators at the Emory Chemical Biology Discovery Center"
Yuhong Du, Ph.D., Asssociate Director of HTS and Assay Development, Emory Chemical Biology Discovery Center; and Assistant Professor of Pharmacology, Emory University
11:45 – 12:45pm Tour of the Emory Chemical Biology Discovery Center
Clinton Maddox, Research Scientist, Emory Chemical Biology Discovery Center, Emory University
11:45 – 12:45pm Lunch (to be provided)
12:45 – 1:00pm Introduction to the GloMax®-Multi+ Detection System
1:00 – 2:30pm GloSensor cAMP Laboratory Activity
2:30 – 2:45pm Break
2:45 – 3:30pm GloResponse NFkappaB Laboratory Activity, Part II
3:30 – 3:45pm Break
3:45 – 4:30pm Tips & Techniques: Getting the Most Out of Your Reporter Assays This short seminar covers practical aspects of luciferase reporter assay design and execution. Topics include:
- Reporter design
- Transfection, cell culture & timing considerations
- Appropriate controls
4:30 – 5:00pm Data Analysis Wrap-up of the day’s events

For more information about Promega's Cell-based Assays, please click here.

Questions?

For Promega, contact Jay Hertweck, Field Applications Specialist
Tel: (678) 296-8746
email: jay.hertweck@promega.com

For ECBDC, contact Cheryl Meyerkord, Ph.D., Administrative Project Manager, ECBDC
Tel: (404) 727-4675
email: emory-ncicbc@pharm.emory.edu